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申永超 林 佳 冯 琪 朱大荣▲.miR-92b与PI3K/Akt在糖尿病肾病中相关性的初步研究[J].中国现代医生,2021,59(30):44-48
miR-92b与PI3K/Akt在糖尿病肾病中相关性的初步研究
The preliminary study on the correlation between miR-92b and PI3K/Akt in diabetic nephropathy
  
DOI:
中文关键词:  糖尿病肾病  miR-92b  PI3K  Akt  LY294002
英文关键词:Diabetic nephropathy  miR-92b  PI3K  Akt  LY294002
基金项目:浙江省医药卫生科技计划项目(2020RC092)
作者单位
申永超 林 佳 冯 琪 朱大荣▲ 杭州市第一人民医院放射科浙江杭州 310006 
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中文摘要:
      目的 探讨miR-92b 与信号通路PI3K/Akt 在糖尿病肾病中的相关性。方法 选取8 周龄的C57BL/6 雄性小鼠12 只,随机分为正常对照组和糖尿病肾病组,利用枸橼酸钠建立糖尿病肾病小鼠模型;选取人肾小管上皮HK-2 细胞12 瓶,随机分为高糖组、低糖组和左旋葡萄糖组,用高糖刺激建立糖尿病肾病细胞模型。利用实时荧光定量PCR 检测小鼠肾脏组织和细胞中miR-92b 表达水平,Western blot 检测小鼠肾脏组织和细胞中Akt 和PAkt 的表达水平。结果 糖尿病肾病组和高糖组miR-92b 表达水平明显低于对照组(P<0.05),而P-Akt/Akt 表达水平明显高于对照组(P<0.05)。将信号通路PI3K/Akt 抑制剂LY294002 加入到高糖诱导的糖尿病肾病细胞模型中,信号通路PI3K/Akt 被抑制的同时,miR-92b 表达显著升高(P<0.05)。结论 糖尿病肾病时,miR-92b 与PI3K/Akt 的表达呈负相关。
英文摘要:
      Objective To explore the relationship between miR-92b and PI3K/Akt signaling pathway in diabetic nephropathy.Methods Twelve 8-week-old male C57BL/6 mice were randomly divided into a normal control group and a diabetic nephropathy group.The diabetic nephropathy mouse model was established using sodium citrate.12 bottles of human renal tubular epithelial HK-2 cells were selected and randomly divided into high glucose group,low glucose group and L-glucose group.The diabetic nephropathy cell model was established with high glucose stimulation.The expression levels of miR-92b in mouse kidney tissues and cells were detected by RT-PCR.The expression levels of Akt and P-Akt in mouse kidney tissues and cells were detected by Western blot.Results Compared with that of the control group,the expression level of miR-92b in the kidney tissue and cells of the diabetic nephropathy model group was significantly lower (P<0.05),and the expression level of P-Akt/Akt was significantly higher(P<0.05).The PI3K/Akt signaling pathway inhibitor LY294002 was added to the high glucose-induced diabetic nephropathy cell model.While PI3K/Akt signaling pathway was inhibited,the expression level of miR-92b was significantly increased (P<0.05).Conclusion In diabetic nephropathy,miR-92b is negatively correlated with the expression of PI3K/Akt signaling pathway.
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