| 王亚萍 余军辉▲ 王 凯.下调ARPC2通过抑制Wnt/β-catenin通路激活水平降低卵巢癌OVCA429细胞恶性转移潜能[J].中国现代医生,2021,59(19):38-43 |
| 下调ARPC2通过抑制Wnt/β-catenin通路激活水平降低卵巢癌OVCA429细胞恶性转移潜能 |
| Down-regulation of ARPC2 reducing the malignant metastatic potential of ovarian cancer OVCA429 cells by inhibiting Wnt/β-catenin pathway |
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| DOI： |
| 中文关键词: 卵巢癌 肌动蛋白相关蛋白复合体2 迁移 Wnt/β-catenin 通路 |
| 英文关键词:Ovarian cancer Actin-related protein complex 2 Migration Wnt/β-catenin pathway |
| 基金项目:浙江省公益技术研究计划项目（LGF19H160019） |
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| 中文摘要: |
| 目的 探讨下调肌动蛋白相关蛋白复合体2（ARPC2）对卵巢癌OVCA429 细胞恶性转移潜能的影响和机制方法 qRT-PCR 和Western blot 检测卵巢癌Skov3、A2780、OVCA429 细胞和正常卵巢IOSE386 细胞中ARPC2 表达变化。在卵巢癌OVCA429 细胞中转染ARPC2 siRNA，MTT 测定细胞增殖，Transwell 小室检测细胞迁移和侵袭，用Western blot 方法检测细胞中ARPC2、β-catenin、c-myc、MMP-9、MMP-2 蛋白表达变化。用Wnt/β-catenin 激活剂LiCl 处理转染ARPC2 siRNA 后的卵巢癌OVCA429 细胞，同样利用上述方法测定细胞增殖、迁移、侵袭变化。结果 卵巢癌Skov3、A2780、OVCA429 细胞中ARPC2 表达水平明显高于正常卵巢IOSE386 细胞。卵巢癌OVCA429 细胞中ARPC2 表达水平最高。转染ARPC2 siRNA 后的卵巢癌OVCA429 细胞增殖能力下降，细胞迁移和侵袭能力也下降，细胞中ARPC2、β-catenin、c-myc、MMP-9、MMP-2 蛋白表达水平均降低。Wnt/β-catenin 激活剂LiCl 可以逆转ARPC2 siRNA 对卵巢癌OVCA429 细胞增殖、迁移和侵袭的抑制作用。结论 下调ARPC2 通过抑制Wnt/β-catenin 通路激活水平降低卵巢癌OVCA429 细胞恶性转移潜能。 |
| 英文摘要: |
| Objective To investigate the effect and mechanism of down-regulation of actin-related protein complex 2（ARPC2）on the malignant metastatic potential of ovarian cancer OVCA429 cells.Methods The changes of APC2 expression in ovarian cancer Skov3，A2780，OVCA429 cells，and normal ovarian IOSE386 cells were detected by qRTPCR and Western blot.Arpc2 siRNA was transfected into ovarian cancer OVCA429 cells.Cell proliferation was determined by MTT.Cell migration and invasion were detected by the Transwell chamber.The ARPC2，β-catenin，c-myc，MMP-9，MMP-2 protein expression in cells were detected by Western blot.The ovarian cancer OVCA429 cells transfected with APC2 siRNA were treated with the Wnt/β-catenin activator LiCl.Cell proliferation，migration，and invasion changes were also measured by the above method.Results The expression level of ARPC2 in ovarian cancer Skov3，A2780，and OVCA429 cells was significantly higher than that of normal ovarian IOSE386 cells.The expression level of ARPC2 was the highest in ovarian cancer OVCA429 cells.After the transfection of APC2 siRNA，the proliferation ability of ovarian cancer OVCA429 cells decreased，and the cell migration and invasion ability also decreased.The expression of APC2，β-catenin，c-myc，MMP-9，MMP-2 protein in the cells decreased.The Wnt/β-catenin activator LiCl can reverse the inhibitory effects of APC2 siRNA on the proliferation，migration，and invasion of ovarian cancer OVCA429 cells.Conclusion Down-regulation of APC2 reduces the malignant metastatic potential of ovarian cancer OVCA429 cells by inhibiting the activation of Wnt/β-catenin pathway. |
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