| Objective To analyze the mechanism of sulodexide in regulating microRNA-27a (miR-27a)-mediated autophagy in the regulation of podocyte injury in diabetic nephropathy.Methods Fifteen of the 45 rats were selected as the normal group without any treatment,and the remaining rats were used to establish a diabetic nephropathy rat model and randomly divided into the model group and the sulodexide group with 15 rats in each group.Rats in the sulodexide group were given intramuscular injection of sulodexide,while those in the normal group and the model group were given intramuscular injection of equal volume of normal saline.Histopathological changes,blood glucose,blood lipid and renal function indexes,podocyte autophagosome status,apoptosis rates,and podocyte specific protein expression levels,including Nephrin,Desmin,GPR124,miR-27a and AMPK-mtor signaling pathway protein were observed.Results The blood glucose,lipid and renal function indexes,podocyte apoptosis rates,Desmin,miR-27a and AMPK-mtor signaling pathway protein expression levels in the sulodexide group were lower than those in the model group,with significant difference (P<0.05).The number of podocyte autophagosomes,the protein expression of Nephrin and GPR124 in the sulodexide group were higher than those in the model group,with significant difference (P<0.05).Conclusion Sulodexide can mediate the reduction of the expression of AMPK,mTOR and Bax proteins by decreasing miR-27a,and then inhibit the AMPK-mTOR signaling pathway,promote autophagy,inhibit apoptosis and reduce the injury of podocytes in diabetic nephropathy. |
