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童巧文1 陈杨芳2 林建萍2 冯 梅1 叶 华1▲.帕金森病患者肠道菌群与血清炎症因子的相关分析[J].中国现代医生,2021,59(14):12-15+封三
帕金森病患者肠道菌群与血清炎症因子的相关分析
Correlation analysis on intestinal flora and serum inflammatory factors in the patients with Parkinson′s disease
  
DOI:
中文关键词:  帕金森病  肠道菌群  炎症因子  相关性分析
英文关键词:Parkinson′s disease (PD)  Intestinal flora  Inflammatory factors  Correlation analysis
基金项目:浙江省医药卫生科技计划项目(2019KY204);浙江省温州市科技计划项目(Y20190557);浙江省温州市科技计划项目(Y20170064)
作者单位
童巧文1 陈杨芳2 林建萍2 冯 梅1 叶 华1▲ 1.浙江省温州市人民医院神经内科浙江温州 3250002.浙江省温州市人民医院检验科浙江温州 325000 
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中文摘要:
      目的 分析帕金森病(PD)患者肠道菌群的改变与血清炎症因子的相关性分析。方法 筛选2017 年11 月至2019 年2 月就诊于温州市人民医院神经内科的原发性PD 患者30 例和同期30 例正常体检者。入组后搜集一般资料,采集血液、粪便样本,通过16S-rRNA 测序确定微生物种类并进行微生物的分类分析,并检测血清脂多糖(LPS)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)。对PD 差异菌群丰度和血清炎症因子进行相关性分析。结果 从属水平上看,HC 组相对于PD 组,毛螺旋菌、链球菌属、克雷伯菌属、伯劳特菌属4 种属显著增加(P<0.05)。PD 组相对于HC 组,有埃希氏菌属、瘤胃球菌属、巨单胞菌属、阿克曼氏菌属、巨型球菌属、颤杆菌克属、伯克氏菌属、埃森博格拉属、异味杆菌属、丹毒丝菌科未命名属、脱硫弧菌属、普雷沃氏菌科、单胞菌属、脱硫弧菌科、嗜胨菌属明显增加(P<0.05)。PD 组血清TNF-α 水平为101.56(46.30,200.62),高于对照组血清TNF-α 水平83.53(37.72,191.27),差异有统计学意义(P<0.05);两组血清IL-6,LPS 水平,差异无统计学意义(P>0.05)。TNF-α、IL-6、LPS 与脱硫化弧菌属(Desulfovibrio)有正相关(P<0.05),IL-6、LPS 与丹毒丝菌科未命名属(Erysipelotrichaceae incertae sedis)有正相关(P<0.05),IL-6、LPS 与伯劳特菌属(Blautia)有正相关(相关系数为P<0.05)。结论 帕金森病组和健康对照组的菌群结构存在有差异。PD 肠道菌群中条件致病菌增多,并与细胞因子有一定相关。这些改变可能与帕金森病的发病机制有关。
英文摘要:
      Objective To analyze the correlation between changes of intestinal flora and serum inflammatory factors in the patients with Parkinson′s disease (PD).Methods A total of 30 patients with primary PD who were admitted to the Department of Neurology in Wenzhou People′s Hospital from November 2017 to February 2019 and 30 patients undergoing routine physical examination during the same period were selected.After grouping,the general information was collected,and the blood and stool samples were collected.The types of microorganisms was determined through 16SrRNA sequencing and the classification analysis of microorganisms was performed.The serum lipopolysaccharide (LPS),interleukin-6 (IL-6),tumor necrosis factor-α (TNF-α) were detected.Correlation Analysis was carried out between different flora abundance and serum inflammatory factors in PD.Results From the genus level,compared with the PD group,the HC group had significant increases in four genera:Lachnospira,Streptococcus,Klebsiella,and Blautia(P<0.05).Compared with the HC group,the PD group had significant increases in Escherichia,Ruminococcus,Megamonas,Akkermansia,Megacoccus,Oscillibacter,Burkholderia,Erysipelotrichaceae incertae sedis,Desulfovibrio,Desulfovibrio,Desulfovibrio,and Peptoniphilus (P<0.05).The serum TNF-α level in the PD group was 101.56 (46.30,200.62),which was higher than the serum TNF-α level of 83.53 (37.72,191.27) in the control group,and the difference was statistically significant(P<0.05).There was no statistical difference in the levels of serum IL-6 and LPS between the two groups(P>0.05).TNF-α,IL-6,and LPS were positively correlated with Desulfovibrio(P<0.05),IL-6,LPS were positively correlated with Erysipelotrichaceae incertae sedis(P<0.05),and IL-6 and LPS were positively correlated with Blautia (P<0.05).Conclusion There are differences in the structure of the flora between the Parkinson′s disease group and the healthy control group.The increase in the intestinal flora of conditional pathogenic bacteria of PD is related to cytokines.These changes may be related to the pathogenesis of Parkinson′s disease.
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